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tlpA [2019-11-11 12:57:00]
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tlpA [2019-11-11 12:57:00]

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein
Locus
BSU_31250
Isoelectric point
4.91
Molecular weight
72.09 kDa
Protein length
662 aa Sequence Blast
Gene length
1989 bp Sequence Blast
Function
control of chemotaxis
Product
methyl-accepting chemotaxis protein
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Membrane-bound chemoreceptors
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,208,280 3,210,268

    The protein

    Paralogous protein(s)

  • TlpB, McpC, McpB, McpA

    • Domains

  • Cache domain (aa 152-228) (according to UniProt)
  • HAMP domain (aa 303-355) (according to UniProt)
  • Methyl-accepting transducer domain (aa 374-610) (according to UniProt)

    • Localization

  • cell membrane PubMed, Polar (Septum) PubMed
  • accumulates at strongly curved membrane areas generated during cell division PubMed

    • Expression and Regulation

    Operons

    Genes
    tlpA-mcpA
    Description
    PubMed

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

    • Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

    • Additional information

  • in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell PubMed
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    Biological materials

    Mutant

  • BKE31250 (tlpA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT, downstream forward: _UP4_TAATAGGTCTAAAATACTAT
  • BKK31250 (tlpA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCCTTTTGAACCTTCT, downstream forward: _UP4_TAATAGGTCTAAAATACTAT

    • References

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